Claudin 6 Recombinant Rabbit mAb (S-521-63)

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货号： S0B0400

Datasheet COA

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Claudin 6
分子别名

Skullin, CLDN6
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P56747
克隆号

S-521-63
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P, ICC, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC	1:500
ICC	1:500

背景介绍

Claudin-6 (CLDN6) is a member of the claudin family and serves as a tight junction molecule, which plays a vital role in cell-to-cell adhesion in epithelial or endothelial cell sheets. CLDN6 has been identified to be the origination of cell adhesion signaling taking part in the regulation of nuclear receptor activity through targeting molecules of the nuclear receptor superfamily and managing their gene expression. In terms of its various characters in human cancers, CLDN6 may be a helpful positive marker for further identification of atypical teratoid/rhabdoid tumors (AT/RTs) for diagnosis and therapy. CLDN6 is also reported to be a possible single prognostic marker and promising therapeutic target for a subgroup of intestinal type gastric cancer.

免疫印迹
- WB result of Claudin 6 Rabbit mAb
  Primary antibody: Claudin 6 Rabbit mAb at 1/1000 dilution
  Lane 1: LNCaP whole cell lysate 20 µg
  Lane 2: JAR whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: OVCAR-3 whole cell lysate 20 µg
  Negative control: LNCaP whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti- Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti- Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti- Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti- Claudin 6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in OVCAR3 cells (top panel) and negative staining in DU-145 cells (below panel). Anti-Claudin 6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).