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Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-510-64) STAR

RAC-alpha serine/threonine-protein kinase,Protein kinase B (PKB),Protein kinase B alpha (PKB alpha),Proto-oncogene c-Akt,RAC-PK-alpha,PKB,RAC,AKT1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0363
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产品介绍

产品介绍 FAQs 引用文献(2) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Akt (Ser473)

  • 分子别名

    RAC-alpha serine/threonine-protein kinase; Protein kinase B (PKB); Protein kinase B alpha (PKB alpha); Proto-oncogene c-Akt; RAC-PK-alpha; PKB; RAC; AKT1

  • 细胞定位

    Cytoplasm, Nucleus, Cell membrane

  • Accession

    P31749

  • 克隆号

    S-510-64

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Ms
背景介绍

  • Akt or Protein kinase B, is a serine/threonine kinase that plays an important role in regulating a number of cellular processes such as growth, metabolism and survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway in human cancers such as the PTEN and PI3-kinase (P110α), which occur in more than 30% of human tumors. For Akt to achieve full activation, phosphorylation is needed at both serine 473 (ser473) of the hydrophobic tail and threonine 308 (thr308) of the activation motif, upon growth factor ligation to the receptor tyrosine kinases.

  • 免疫印迹

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HeLa starve overnight, then treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-Akt (Ser473) Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: C6 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 免疫沉淀

    • Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg (Input)
      Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

  • 斑点杂交

    • Dot blot result of Phospho-Akt (Ser473) Rabbit mAb
      Lane 1: phospho-Akt (Ser473) peptide
      Lane 2: Akt peptide
      Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

引用文献(2)

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