SLC7A5/LAT1 Recombinant Rabbit mAb (S-367-12)

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货号： S0B0277

Datasheet COA

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

SLC7A5/LAT1
分子别名

Large neutral amino acids transporter small subunit 1, 4F2 light chain (4F2 LC; 4F2LC), CD98LC, MPE16, hLAT1, y+ system cationic amino acid transporter
免疫原

Synthetic Peptide
细胞定位

Membrane, Lysosome
Accession

Q01650
克隆号

S-367-12
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC	1:2000
ICC	1:500

背景介绍

SLC7A5 (Large neutral amino acids transporter small subunit 1), also known as 4F2 light chain, or CD98 light chain is a protein that in humans is encoded by the SLC7A5 gene. It is suggested to play a part in altered cell metabolism and proliferative signaling and has been reported to be overexpressed in various types of cancer, including breast cancer [PMID: 34792178].

免疫印迹
- WB result of SLC7A5/LAT1 Rabbit mAb Primary antibody: SLC7A5/LAT1 Rabbit mAb at 1/1000 dilution Lane 1: LNCaP whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: HT-1080 whole cell lysate 20 µg Lane 4: MCF7 whole cell lysate 20 µg Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 36 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SLC7A5/LAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti-SLC7A5/LAT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
- Negative control：ICC shows negative staining in LNCAP cells. Anti-SLC7A5/LAT1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).