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宿主来源
Rabbit抗原名称
PI3 Kinase p85α分子别名
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1免疫原
Synthetic Peptide细胞定位
Membrane, Cytoplasm, NucleusAccession
P27986克隆号
S-328-24抗体类型
Rabbit mAb应用
ICFCM, IHC-P, ICC, WB, IP反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Or, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| IHC | 1:250 |
| ICFCM | 1:500 |
| ICC | 1:500 |
Cell proliferation and many other cell processes can be regulated through a signaling pathway that involves an enzyme called PI3K. This ‘heterodimeric’ enzyme is made up of two protein subunits, one of which is called p85α and inhibits the other subunit of the enzyme (known as p110) to prevent uncontrolled cell proliferation. Activation of the class 1A PI3K is gated, in part, by the p85α regulatory subunit, which contains a Src homology 3 (SH3) domain, two proline-rich (PR) regions (PR1 and PR2) separated by a Rho-GAP/BCR-homology (BH) domain, and two Src homology 2 (SH2) domains (nSH2, cSH2) flanking an inter-SH2 (iSH2) domain [PMID: 26222500].
免疫印迹
WB result of PI3 Kinase p85α Rabbit mAb Primary antibody: PI3 Kinase p85α Rabbit mAb at 1/1000 dilution Lane 1: HepG2 whole cell lysate 20 µg Lane 2: MCF7 whole cell lysate 20 µg Lane 3: Jurkat whole cell lysate 20 µg Lane 4: Raji whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 85 kDa Observed MW: 85, 55, 43 kDa
WB result of PI3 Kinase p85α Rabbit mAb Primary antibody: PI3 Kinase p85α Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Lane 2: RAW 264.7 whole cell lysate 20 µg Lane 3: PC-12 whole cell lysate 20 µg Lane 4: mouse brain lysate 20 µg Lane 5: mouse heart lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 85 kDa Observed MW: 85, 43 kDa
WB result of PI3 Kinase p85α Rabbit mAb Primary antibody: PI3 Kinase p85α Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Lane 2: rat heart lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 85 kDa Observed MW: 85, 43 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte) labelling PI3 Kinase p85α antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control. Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
PI3 Kinase p85α Rabbit mAb at 1/50 dilution (1µg) immunoprecipitating PI3 Kinase p85α in 0.4 mg Raji whole cell lysate. Western blot was performed on the immunoprecipitate using PI3 Kinase p85α Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: Raji whole cell lysate 20 µg (Input) Lane 2: PI3 Kinase p85α Rabbit mAb IP in Raji whole cell lysate Lane 3: Rabbit monoclonal IgG IP in Raji whole cell lysate Predicted MW: 85 kDa Observed MW: 85,55,43 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- PI3 Kinase p85α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Raji cells. Anti-PI3 Kinase p85α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
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