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Rabbit抗原名称
CD3 EPSILON分子别名
T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3E, CD3E免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P07766克隆号
S-241-49抗体类型
Recombinant mAb抗体同种型
IgG应用
IF反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:200 |
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon (A647 conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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