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Rabbit抗原名称
Helicobacter pylori分子别名
Hp, H. pylori, Campylobacter pylori克隆号
S-R273抗体类型
Recombinant mAb抗体同种型
IgG应用
IF ?反应种属 ?
Helicobacter pylori纯化方式
Protein A浓度
1 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:100 |
Helicobacter pylori, previously known as Campylobacter pylori, is a gram-negative, microaerophilic, spiral (helical) bacterium usually found in the stomach. Its helical shape (from which the genus name, helicobacter, derives) is thought to have evolved in order to penetrate the mucoid lining of the stomach and thereby establish infection. H. pylori has been associated with cancer of the mucosa-associated lymphoid tissue in the stomach, esophagus, colon, rectum, or tissues around the eye (termed extranodal marginal zone B-cell lymphoma of the cited organ), and of lymphoid tissue in the stomach (termed diffuse large B-cell lymphoma). H. pylori infection usually has no symptoms but sometimes causes gastritis (stomach inflammation) or ulcers of the stomach or first part of the small intestine. The infection is also associated with the development of certain cancers.
免疫荧光
IF shows positive staining in paraffin-embedded human stomach (Hp+). Anti-Helicobacter pylori antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human stomach (Hp-). Anti-Helicobacter pylori antibody was used at 1/100 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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