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Rabbit抗原名称
β-tubulin分子别名
TUBB, TUBB5, Tubulin beta-5 chain免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P07437克隆号
S-312-113抗体类型
Recombinant mAb应用
ICFCM, ICC反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Dr, Lob, Pz, Ar, Av, Pl, Fu, Ys, SeUr, Bv, Pg, Fs纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:200 |
| ICFCM | 1:2000 |
α- and β-tubulin polymerize into dynamic microtubules. In eukaryotes, microtubules are one of the major components of the cytoskeleton, and function in many processes, including structural support, intracellular transport, and DNA segregation. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. The β-tubulin subunit is exposed on the plus end of the microtubule, while the α-tubulin subunit is exposed on the minus end. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the β-tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament.
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling β-tubulin (Alexa Fluor® 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling β-tubulin (Alexa Fluor® 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling β-tubulin (Alexa Fluor® 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-β-tubulin (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-β-tubulin (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti-β-tubulin (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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