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Progesterone Receptor Recombinant Rabbit mAb (Alex Fluor®647 Conjugate) (S-R123)

PR,NR3C3

价格 ￥1,056.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0134
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Progesterone Receptor

  • 分子别名

    PR, NR3C3

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P06401

  • 克隆号

    S-R123

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Alexa Fluor® 647

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

  • 应用

    ICFCM

    ICC ?

    IF ?

  • 稀释度

    应用 稀释度
    ICC 1:500
    IF 1:500
    ICFCM 1:2000
背景介绍

  • Progesterone receptor (PR) is a master regulator in female reproductive tissues that controls developmental processes and proliferation and differentiation during the reproductive cycle and pregnancy. PR also plays a role in progression of endocrine-dependent breast cancer. As a member of the nuclear receptor family of ligand-dependent transcription factors, the main action of PR is to regulate networks of target gene expression in response to binding its cognate steroid hormone, progesterone [PMID: 27380738].

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, left) / T-47D (Human ductal breast epithelial tumor epithelial cell, right) cells labelling Progesterone Receptor (Alexa Fluor 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: MDA-MB-231

  • 免疫细胞化学

    • ICC shows positive staining in T-47D cells. Anti- Progesterone Receptor (Alex Fluor®647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Progesterone Receptor (Alex Fluor®647 Conjugate) antibody was used at 1/500 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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