Akt (pan) Recombinant Rabbit mAb (SDT-R169)

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货号： S0B0114

Datasheet COA

价格： ￥600
规格：
25ul50ul100μl1ml
数量：

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

AKT1 + AKT2 + AKT3
分子别名

N/A
免疫原

N/A
细胞定位

Cell membrane, Cytoplasm, Nucleus
Accession

P31749, Q9Y243, P31751
克隆号

SDT-R169
抗体类型

Recombinant mAb
应用

IHC-P, WB, IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标签

N/A
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500-1:2000
IP	1:50

背景介绍

Akt is a family of three serine-threonine kinases, Akt1, Akt2, and Akt3 [PMID: 28115590]. Akt is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. The Akt can be activated by a wide range of growth signals and the biochemical mechanisms leading to Akt activation are well defined [PMID: 31173856].

免疫印迹
- WB result of Akt (pan) Rabbit mAb
  Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60kDa
  Observed MW: 60kDa
- WB result of Akt (pan) Rabbit mAb
  Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60kDa
  Observed MW: 60kDa
- WB result of Akt (pan) Rabbit mAb
  Primary antibody: Akt (pan) Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60kDa
  Observed MW: 60kDa
免疫沉淀
- Akt (pan) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Akt (pan) in 0.4 mg MCF-7 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Akt (pan) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: MCF-7 whole cell lysate 20 µg (Input)
  Lane 2: Akt (pan) Rabbit mAb IP in MCF-7 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in MCF-7 whole cell lysate
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Akt (pan) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Akt (pan) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.