alpha smooth muscle Actin Recombinant Mouse mAb (SDT-R142)

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货号： S0B0102

Datasheet COA

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

宿主来源

Mouse
抗原名称

ALPHA SMOOTH MUSCLE ACTIN
分子别名

Alpha-actin-2, ACTA2, SMA
免疫原

N/A
细胞定位

Cytoplasm, Cytoskeleton
Accession

P62736
克隆号

SDT-R142
抗体类型

Recombinant mAb
抗体同种型

IgG2a
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.4 mg/ml
标签

N/A
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000-1:4000
IHC-P	1:4000
ICC	1:100
ICFCM	1:400
IP	1:40

背景介绍

α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

免疫印迹
- WB result of alpha smooth muscle Actin mouse mAb
  Primary antibody: alpha smooth muscle Actin mouse mAb at 1/4000 dilution
  Lane 1: mouse skeletal muscle lysate 5 µg
  Lane 2: mouse stomach lysate 5 µg
  Negative control: mouse skeletal muscle lysate
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42kDa
  Observed MW: 42kDa
- WB result of alpha smooth muscle Actin mouse mAb
  Primary antibody: alpha smooth muscle Actin mouse mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42kDa
  Observed MW: 42kDa
流式分析
- Flow cytometric analysis of HeLa cells labelling alpha smooth muscle Actin antibody at 1/400 (0.1 μg) dilution/ (red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- alpha smooth muscle Actin Mouse mAb at 1/40 dilution (1 µg) immunoprecipitating alpha smooth muscle Actin in 0.4 mg NIH/3T3 whole cell lysate.
  Western blot was performed on the immunoprecipitate using alpha smooth muscle Actin Mouse mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
  Lane 2: alpha smooth muscle Actin Mouse mAb IP in NIH/3T3 whole cell lysate
  Lane 3: Mouse monoclonal IgG2a IP in NIH/3T3 whole cell lysate
  Predicted MW: 42 kDa
  Observed MW: 42 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI. Counterstain with tubulin (red).

引用文献(1)

Procyanidin C1 ameliorates aging-related skin fibrosis through targeting EGFR to inhibit TGFβ/SMAD pathway
JH Wang, M Li, PF Xie, JY Si, ZJ Feng, CF Tang, JM Li

Phytomedicine. 2025 Apr 17 ; 1

影响因子： 6.7

货号：S0B0102产品名称：alpha smooth muscle Actin Recombinant Mouse mAb (SDT-R142)