alpha smooth muscle Actin Recombinant Rabbit mAb (Alexa Fluor^® 488 Conjugate) (SDT-132-63)

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货号： S0B0060

大包装询价

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产品规格

稀释度

应用	稀释度
IF	1:500

背景介绍

α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

免疫细胞化学
- IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.