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Rabbit抗原名称
S100A8 (MRP8)分子别名
Protein S100-A8, CFAG免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, SecretedAccession
P05109克隆号
SDT-059-28抗体类型
Rabbit mAb应用
IHC-P, ICC, WB, IP反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05 %BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:2000 |
| IP | 1:50 |
| ICC | 1:500 |
S100A8 (MRP8) is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.
免疫印迹
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/205e5046da8f46c3944a6035c84477e3.jpg)
WB result of S100A8 (MRP8) Rabbit mAb
Primary antibody: S100A8 (MRP8) Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 10 kDa
Exposure time: 10s
免疫沉淀
![item.picture[0].fileOrgName](https://univ-pmc.oss-cn-shanghai.aliyuncs.com/product.imager.attribute/0/50e6c907-9367-4df5-a758-1358bb0fbd63.jpg)
S100A8 (MRP8) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating S100A8 (MRP8) in 0.4 mg HL-60 whole cell lysate.
Western blot was performed on the immunoprecipitate using S100A8 (MRP8) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HL-60 whole cell lysate 20 µg (Input)
Lane 2: S100A8 (MRP8) Rabbit mAb IP in HL-60 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HL-60 whole cell lysate
Predicted MW: 10 kDa
Observed MW: 12 kDa
Exposure time: 39 s
免疫组化
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/60da091eccbd4dd68bdf44f46afb972d.jpg)
IHC shows positive staining in paraffin-embedded human spleen. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/9e79c295ea464497a4ef59628d670b16.jpg)
IHC shows positive staining in paraffin-embedded human tonsil. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/f8d238e09cdc46adb412afb776cacac0.jpg)
IHC shows positive staining in paraffin-embedded human tonsil. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/d279e1dc9152419bb5e8f09151628f5d.jpg)
IHC shows positive staining in paraffin-embedded human Hodgkin's lymphoma. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/777433e81e2e4639a0feb317d876c4fa.jpg)
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/3a6f038c9d3148e38bf73b106323317c.jpg)
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/f57ef58c678c411ba715522d64736661.jpg)
IHC shows positive staining in paraffin-embedded rat spleen. Anti-S100A8 (MRP8) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
![item.picture[0].fileOrgName](https://univ-pmc.oss-cn-shanghai.aliyuncs.com/product.imager.attribute/0/96d2904e-cece-4c32-880c-0174afa695f0.jpg)
ICC shows positive staining in SK-BR-3 cells (top panel) and negative staining in 293T cells (below panel). Anti-S100A8 (MRP8) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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