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NA/LE Mouse anti-human CD28 mAb

TP44

价格 ￥1,200.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0010
规格
数量
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产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    CD28

  • 分子别名

    TP44

  • 免疫原

    N/A

  • Accession

    P10747

  • 克隆号

    15E8

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 同型对照

    Invivo mouse IgG1 isotype control

  • 应用

    Functional Assay ,
    in vitro T cell stimulation/activation ,
    FCM

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein G

  • 浓度

    Lot specific* (generally 0.5 to 10 mg/ml)*

  • 纯度

    >95%(Determined by SDS-PAGE)

  • 内毒素含量

    ＜1EU/mg

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4, containing no preservative

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;

    -20 °C for 3 months under sterile conditions;

    -80 °C for 24 months under sterile conditions.

    Please avoid repeated freeze-thaw cycles.

稀释度
应用 稀释度
Functional Assay 1:500
FCM 1:500-1000
背景介绍

  • This antibody recognizes the same epitope as clone 15E8.

    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular).
    CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.

  • 组分和操作说明

    • Procedure

      1. Isolate human PBMCs according to the manufacturer’s instructions.

      2. Suspend PBMCs in medium at 106 cells/mL.

      3. Do one of the following depending on the format you are using:

      · For the soluble format, incubate 106 cells with CD3 Mouse mAb (OKT3) at 1 µg/mL final concentration in culture for 3 days (5% CO2, 37°C).

      · For the immobilized format, coat the CD3 Mouse mAb (OKT3) onto a plate at 5 to 10 μg/mL at 4°C, overnight. Add cells to the CD3 Mouse mAb (OKT3)-coated plate.

      4. Wash the cells twice and resuspend them in medium at 106 cells/mL.

      5. Distribute the cells in a round-bottom plate at 105 cells per well.

      6. [Optional for co-stimulation] Add CD28 Mouse mAb (15E8) and optionally Protein G at 5 µg/mL each final concentration, and incubate the plate for an additional 3 days (5% CO2 at 37°C).

  • 验证数据

    • Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, right) cells labelling CD28 antibody at 1/500 dilution (0.1 μg)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: THP-1

    • T cell proliferation Assay with CD3(S0B0009) &CD28 (S0B0010)

    • T cell proliferation Assay with CD3(S0B0009) &CD28 (S0B0010)

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