热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 功能性抗体

所有图片（1/4）

NA/LE Mouse anti-human CD3 mAb

T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,T3E

价格 ￥960.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0009
规格
数量
收藏 分享

关联产品推荐

  • NA/LE Mouse anti-human CD28 mAb

    规格：1mg

    价格：￥1,200.00

    • 产品介绍 引用文献(1) 评论(0)

    产品规格

    • 宿主来源

      Mouse

    • 抗原名称

      CD3

    • 分子别名

      T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3E

    • 免疫原

      N/A

    • Accession

      P07766

    • 克隆号

      OKT3

    • 抗体类型

      Mouse mAb

    • 抗体同种型

      IgG2a,k

    • 同型对照

      Invivo mouse IgG2a isotype control

    • 应用

      Functional Assay ,
      in vitro T cell stimulation/activation ,
      FCM

    • 反应种属 ?

      Hu

    • 纯化方式

      Protein A

    • 浓度

      Lot specific* (generally 0.5 to 5 mg/ml)*

    • 纯度

      >95% (Determined by SDS-PAGE)

    • 内毒素含量

      ＜1EU/mg

    • 标记

      Unconjugated

    • 性状

      Liquid

    • 缓冲体系

      PBS pH7.4, containing no preservative

    • 储存条件

      2 to 8 °C for 2 weeks under sterile conditions;

      -20 °C for 3 months under sterile conditions;

      -80 °C for 24 months under sterile conditions.

      Please avoid repeated freeze-thaw cycles.

    稀释度
    应用 稀释度
    Functional Assay 1:500
    FCM 1:500
    背景介绍

    • This antibody recognizes the same epitope as clone OKT3.

      CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.

    • 组分和操作说明

      • Procedure

        1. Isolate human PBMCs according to the manufacturer’s instructions.

        2. Suspend PBMCs in medium at 106 cells/mL.

        3. Do one of the following depending on the format you are using:

        · For the soluble format, incubate 106 cells with CD3 Mouse mAb (OKT3) at 1 µg/mL final concentration in culture for 3 days (5% CO2, 37°C).

        · For the immobilized format, coat the CD3 Mouse mAb (OKT3) onto a plate at 5 to 10 μg/mL at 4°C, overnight. Add cells to the CD3 Mouse mAb (OKT3)-coated plate.

        4. Wash the cells twice and resuspend them in medium at 106 cells/mL.

        5. Distribute the cells in a round-bottom plate at 105 cells per well.

        6. [Optional for co-stimulation] Add CD28 Mouse mAb (15E8) and optionally Protein G at 5 µg/mL each final concentration, and incubate the plate for an additional 3 days (5% CO2 at 37°C).

    • 验证数据

      • Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labeling CD3 with purified antibody at 1/500 dilution (0.1 μg) (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.

        Cells were surface stained with CD19-Alexa Fluor® 647, then stained with rabbit IgG (Left) / anti-CD3 (Right) separately. CD3 and CD19 are mutually exclusive expressed in human PBMCs. Gated on total viable cells.

      • T cell proliferation Assay with CD3(S0B0009) &CD28 (S0B0010)

      • T cell proliferation Assay with CD3(S0B0009) &CD28 (S0B0010)

      引用文献(1)

      评论(0)