VISTA Recombinant Rabbit mAb (S-1013-5)

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货号： S0B0775

Datasheet COA

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

VISTA
分子别名

V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1 (Sisp-1), V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, VSIR, C10orf54, SISP1
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q9H7M9
克隆号

S-1013-5
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P, WB, IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:250
IP	1:50

背景介绍

The VISTA protein, also known as V-domain immunoglobulin suppressor of T cell activation, plays a crucial role in immune regulation. The VISTA protein primarily functions as a T cell inhibitory ligand and receptor, participating in immune regulation by directly suppressing T cell function. As a co-inhibitory ligand, VISTA can act on yet undefined T cell-expressed receptors to inhibit the proliferation of CD4+ and CD8+ T cells, the production of IL-2 and IFN-γ, and promote the conversion of naive T cells into FoxP3+ regulatory T cells. As a co-inhibitory receptor, VISTA can suppress T cell function independently of antigen-presenting cells (APCs). VISTA-deficient mice exhibit spontaneous T cell activation, high levels of proinflammatory cytokine secretion, and more severe experimental autoimmune encephalomyelitis.

免疫印迹
- WB result of VISTA Recombinant Rabbit mAb
  Primary antibody: VISTA Recombinant mAb at 1/1000 dilution
  Lane 1: MCF-7 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: DU 145 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Negative control: MCF-7 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 34 kDa
  Observed MW: 38~70 kDa
免疫沉淀
- VISTA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VISTA in 0.4 mg DU 145 whole cell lysate.
  Western blot was performed on the immunoprecipitate using VISTA Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: DU 145 whole cell lysate 20 µg (Input)
  Lane 2: VISTA Rabbit mAb IP in DU 145 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in DU 145 whole cell lysate
  Predicted MW: 34 kDa
  Observed MW: 38~70 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VISAT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-VISAT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-VISAT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VISAT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-VISAT antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.