SARS-CoV-2 (BA.2.12.1/Omicron) S protein, His Tag

Product Introduction

• Species

  SARS-CoV-2

• Synonyms

  Spike glycoprotein, S glycoprotein, E2, Peplomer protein

• Accession

  P0DTC2

• Amino Acid Sequence

  Protein sequence(P0DTC2, Val16-Pro1213, with C-10*His) VNLITRTQSYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLGRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFDEVFNATRFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKVGGNYNYQYRLFRKSNLKPFERDISTEIYQAGNKPCNGVAGFNCYFPLRSYGFRPTYGVGHQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRAAASVASQSIIAYTMSLGAENLVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPGGGGSHHHHHHHHHH

• Expression System

  HEK293

• Molecular Weight

  Theoretical:134.4kDa Actual:150kDa

• Purity

  >95% by SDS-PAGE

• Conjugation

  Unconjugated

• Tag

  His Tag

• Physical Appearance

  Lyophilized Powder

• Reconstitution

  Reconstitute no more than 0.1 mg/mL according to the size in deionized water after rapid centrifugation.

• Stability & Storage

  12 months from date of receipt, -20 ℃ to -70 °C as supplied.
  1 month, 2 to 8 °C under sterile conditions after reconstitution.  
  Please avoid repeated freeze-thaw cycles. 

• dilution

• Spike (S) glycoprotein is the largest of the four major structural proteins found in coronaviruses.The spike protein assembles into trimers that form large structures, called spikes or peplomers,that project from the surface of the virion.The function of the spike glycoprotein is to mediate viral entry into the host cell by first interacting with molecules on the exterior cell surface and then fusing the viral and cellular membranes. Spike glycoprotein is a class I fusion protein that contains two regions, known as S1 and S2, responsible for these two functions.

• Bioactivity

  • 

    Immobilized SARS-CoV-2 (BA.2.12.1/Omicron) S protein, His Tag at 4 μg/mL (50 μL/well) can bind Human ACE2, hFc tag (S0A0071) with EC50 of 7.713-10.39 ng/ml.

• SDS-PAGE

  • 
    2μg R: reducing conditions