SARS-CoV-2 (BA.2&BA.5/Omicron) Nucleocapsid, His Tag

Product Introduction

• Species

  SARS-CoV-2

• Accession

  P0DTC9

• Amino Acid Sequence

  MSDNGPQNQRNALRITFGGPSDSTGSNQNGGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSRADSTQAGGGSHHHHHHHH

• Expression System

  E.coli

• Molecular Weight

  48-50kDa(Reducing)

• Purity

  ＞95%SDS-PAGE&RP-HPLC

• Endotoxin

  <1EU/μg

• Conjugation

  Unconjugated

• Physical Appearance

  Lyophilized Powder

• Storage Buffer

  PBS, pH7.4

• Reconstitution

  Reconstitute at less than 1mg/ml according to the size in ultrapure water after rapid centrifugation.

• Stability & Storage

  Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• dilution

• Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has continued to adapt for human infection and transmission, resulting in several variants of concern. While most mutations occur within a single lineage, a small number are shared across multiple variants. The SARS-CoV-2 nucleocapsid (N) gene is one hotspot for coding mutations. Nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

  The mutations are identified on the SARS-CoV-2 Omicron variant Pango lineage BA.4, which included P13L, del31-33, R203K, G204R, S413R.

• SDS-PAGE

  • 
    2μg（R: reducing conditions, N: non-reducing conditions).

• RP-HPLC

  • 
  •